Spherical gold nanoparticles with a particle size of less than 12 nm were prepared by in situ reduction of chloroauric acid with GAGAGAGY, a peptide derived from silk fibroin. The reaction process and product were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy, and the influence of preparative conditions on the product was explored. The results showed that the peptide could act as a stabilizer, endowing the synthesized gold nanoparticles with good stability. When the molar ratio of the polypeptide to chloramic acid is 10 to 4, the size of particles obtained will be uniform. With the increment of alkaline of the solution, the reaction slowed down, but the particle size of the gold nanoparticles was not affected. The rising of temperature could accelerate the reaction, as evidenced by a blue shift of the absorption peak of the reaction solution. |