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| 利用基因工程菌从贵金属废液中吸附铂和钯 |
| Adsorption of Platinum and Palladium from Precious Metal Waste Liquid by Genetic Engineering Bacteria |
| 投稿时间：2019-09-06 |
| DOI： |
| 中文关键词: 表面展示技术 生物吸附铂和钯 膜分离 贵金属废水 |
| 英文关键词: surface display technology biosorption of platinum and palladium membrane separation precious metal wastewater |
| 基金项目:国家自然基金面上项目(51871250，51504106)、稀贵金属综合利用新技术国家重点实验室开放课题(SKL-SPM-201809)、省部共建华南应用微生物国家重点实验室开发课题(SKLAM004-2016)、云南科技计划项目(2015FB204,2016BA006, 2017FA030) |
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| 中文摘要: |
| 采用表面展示技术将含EC20序列的载体转化至E.coli BL21胞内，制备了高效基因工程菌吸附剂(简称基因工程菌)，并利用该工程菌从工业贵金属废液中吸附铂和钯。结果表明，经过表面展示的基因工程菌a对铂和钯的吸附量较E.coli BL21分别提升了1.6倍和1.31倍；当基因工程菌a的添加量为8 g/L，吸附时间为3 h时，工业废水中铂和钯的回收率分别达到90.71%和100%；对比了膜分离与高速离心分离效果，当菌浓度为2 g/L，吸附时间为30 min时，用膜分离的方法所得铂和钯的回收率分别为64.99%和90.09%，是高速离心分离法回收率的2.77倍和1.05倍。 |
| 英文摘要: |
| The surface-display technology was used to transform the vector containing EC20 sequence into the E. coli BL21 cells to prepare a highly efficient genetic engineering bacteria adsorbent (hereinafter referred to as: genetically engineered bacteria), and the engineering bacteria were used to adsorb platinum and palladium from industrial precious metal waste liquid. The results showed that the adsorbed platinum and palladium by genetically engineered bacteria a was 1.6 times and 1.31 times higher than that of E. coli BL21. When the genetically engineered bacteria a was 8 g/L, the adsorption time was 3h, the recovery rates of platinum and palladium in industrial wastewater reached 90.71% and 100%, respectively. The membrane separation and high-speed centrifugal separation were compared. When the concentration of bacteria was 2 g/L and the adsorption time was 30 min, the recovery rates of platinum and palladium by the method were 64.99% and 90.09%, respectively, which were 2.77 times and 1.05 times higher than the high-speed centrifugation. |
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