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| 柠檬酸钠-施氏假单胞菌还原制备纳米银及抑菌活性研究 |
| Preparation of nano-silver by sodium citrate - |
| Received:July 15, 2022 |
| DOI： |
| 中文关键词: 纳米银 制备 施氏假单胞菌 柠檬酸钠 抑菌性 |
| 英文关键词: nano-silver preparation pseudomonas stutzeri sodium citrate antibacterial activity |
| 基金项目:河南省科学院科研开发专项(200605087，220605087) |
| Author Name | Affiliation | | GUO Wenyang | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | ZHOU Liuzhu | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | YUE Dandan | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | PAN Mengshi | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | ZHANG Zongyuan | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | WANG Xueyan | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | QI Landa | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China | | ZHANG Yingtao | Henan Academy of Science Institute of Biology Co. Ltd., Zhengzhou 450008, China |
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| 中文摘要: |
| 以硝酸银为原料，利用柠檬酸钠联合施氏假单胞菌还原法制备纳米银，并对纳米银的紫外吸收特性、微观形貌、物相组成，以及其稳定性和抑菌性进行了分析。结果表明，与单独柠檬酸钠制备的纳米银相比，联合还原法制备的纳米银有明显的紫外吸收峰，其粒径的整体大小减小了约10倍，稳定性同步增强，制备效率提高了26.7%；抑菌实验表明，联合法制备的纳米银对金黄色葡萄球菌和大肠杆菌的抑菌性分别提高了62.5%和77.5%。 |
| 英文摘要: |
| Nano-silver was prepared by sodium citrate combined with Pseudomonas stutzeri reduction method using silver nitrate as the starting material. The ultraviolet absorption characteristics, microscopic morphology, phase composition, stability and antibacterial properties of nano-silver were analyzed. The results showed that compared with the nano-silver prepared by sodium citrate alone, the nano-silver prepared by the combined reduction method exhibited an obvious ultraviolet absorption peak and the overall size of the particle size was reduced by about 10 times and the stability was also enhanced. In addition, the preparation efficiency was improved. More importantly, its antibacterial activity against Staphylococcus aureus and Escherichia coli increased by 62.5% and 77.5%, respectively. |
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